cas9 plasmid dna  (Thermo Fisher)

Journal: Scientific Reports

Article Title: CRISPR/Cas9-mediated deletion of MADD induces cell cycle arrest and apoptosis in anaplastic thyroid cancer cells

doi: 10.1038/s41598-025-22907-1

Figure Lengend Snippet: Construction and characterization of CRISPR/Cas9-mediated genomic knockout of MADD gene in ATC Cells: ( A ) Schematic diagram of the construction of CRISPR/Cas9- MADD dual-sgRNA targeting Exon 3 of the human MADD gene. ( B ) Confirmation of MADD Knockout by PCR compared to control cells. ( C ) Western blot analysis of MADD expression in control and knockout cells after 24 h of treatment with 4-Hydroxytamoxifen (4HT). β-actin served as a loading control. ( D ) Immunocytochemistry of MADD expression. Cells were cultured on sterile coverslips in the 8-well chamber, and following fixation, the cells were stained with anti-MADD-specific antibodies. DAPI staining was used for counter-staining. (Green = MADD, blue = nucleus) absence of any green color indicates that MADD was successfully knocked out in ATC cells at the translational level.

Article Snippet: Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) and sgRNA 1&2 and Cas9 plasmid DNA were separately diluted in 250 μl of portions of Opti-MEM reduced serum medium (Invitrogen).

Techniques: CRISPR, Knock-Out, Control, Western Blot, Expressing, Immunocytochemistry, Cell Culture, Sterility, Staining

Journal: eLife

Article Title: Single-cell eQTL mapping in yeast reveals a tradeoff between growth and reproduction

doi: 10.7554/eLife.95566

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , p415 GalL-Cas9-Cyc1t (plasmid) , , RRID: Addgene_43804 , Gal inducible CAS9 with LEU cassette.

Techniques: Recombinant, Plasmid Preparation, Marker, RNA Expression, Variant Assay, Software